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To generate the respective peptide library initiated with ClAc-L-Tyr (L-library) or ClAc-D-Tyr (D-library), 1.4 M mRNApuromycin library was translated in a methionine-deficient FIT system 14 at a scale of 150 l total volume in the presence of 50 M ClAc-L-Tyr-tRNA fMet CAU or ClAc-D-Tyr-tRNA fMet CAU for 30 min at 37 C

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Treatment with the selective GLS1 inhibitor BPTES reduced glycolysis and ameliorated lupus-like disease and EAE in MRL/lpr mice ( + CD4 - CD8 - cells), the urinary albumin/creatinine ratio, glomerular nephropathy score, and T-cell infiltration with IL-17A in the kidneys, relieving the EAE disease activity ( + T cells by inhibiting GLS1, glycolysis, and CD4 + T-cell differentiation in vitro by reducing the level of HIF1 protein, a key metabolic sensor in Th17 cells ( Table 1 )

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